Application

melanin production, melanocyte proliferation, differentiation

Cell Line Origin

Skin

Components

Basal Medium containing 30% FBS & 10% DMSO

General description

Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.

Preparation Note

2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO Can be cultured at least 12 doublings

Subculture Routine

Please refer to the HEM Culture Protocol.